Spatial patterning of gene expression using surface-immobilized recombinant adenovirus
نویسندگان
چکیده
منابع مشابه
Spatial patterning of gene expression using surface-immobilized recombinant adenovirus.
Spatially patterned gene expression drives tissue organization and is a critical determinant of tissue function. Approaches in functional tissue engineering will require not only the spatial organization of cells but also control of their gene expression patterns. We report a method to generate patterns of gene expression within a monolayer of cells by using surface-immobilized recombinant aden...
متن کاملProduction of Recombinant Adenovirus Containing Human Interlukin-4 Gene
Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...
متن کاملExpression of hepatitis B surface antigen with a recombinant adenovirus.
Early region 1 of the adenovirus type 5 genome was replaced with a DNA sequence containing the gene coding for the hepatitis B surface antigen (HBsAg) flanked by the major late promoter from adenovirus 2 and processing and polyadenylylation signals from simian virus 40. In one type of hybrid virus only the adenovirus 2 major late promoter, including just 33 base pairs of the adenovirus type 2 t...
متن کاملExpression of Recombinant Factor IX Using the Transient Gene Expression Technique
Background: Pilot and large-scale production of recombinant proteins requires the presence of stable clones capable of producing large quantities of recombinant proteins. Not only the process of selecting stable clones is time consuming, but also the continuous culturing of clones in large-scale production may cause loss of incoming plasmid and recombinant genes. Thus, considering the advanceme...
متن کاملproduction of recombinant adenovirus containing human interlukin-4 gene
objective(s) recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. in the present experiment, a reco...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Microdevices
سال: 2008
ISSN: 1387-2176,1572-8781
DOI: 10.1007/s10544-008-9166-7